Melatonin
$98.00
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Questions
Melatonin Common Questions
What’s pIC50?
pIC50 is the negative log of the IC50 value when converted to molar.That is, pIC50=-log(IC50).
What’s Kd?
Dissociation consent (Kd) reflects the affinity of a compound for its target. In some cases, it can be equivalent to Ki.
Product Introduction
Bioactivity
Description
Melatonin (Melatonine) is a natural hormone secreted by the pineal gland that activates melatonin receptors. Melatonin is a hormone that regulates the biological clock and also has antioxidant and anti-inflammatory activities.
In vitro
METHODS: Ten cells were pretreated with EIPA (50 µM) for 1.5 h. Dextran index was measured by Dextran uptake assay.
RESULTS: Immortalized but untransformed hTERT-HME1 mammary epithelial cells and MCF10A cells did not exhibit megaloblast efflux in complete medium but stimulated dextran uptake by nutrient deprivation. Although PIK3CB was found to be required for growth factor-stimulated macrocytosis, oncogenic mutations in PIK3CA were sufficient to induce constitutive macrocytosis in mouse embryonic fibroblast MEFs and untransformed MCF10A cells. [1]
METHODS: MKN28 cells were treated with EIPA (5-100 µM) for 48 h. Cell proliferation was detected by cell count.
RESULTS: Cell exposure to EIPA inhibited the proliferation of MKN28 cells in a dose- and time-dependent manner. [2]
In vitro
METHODS: To assay in vivo activity, EIPA (10 mg/kg) was injected intraperitoneally into BALB/c mice bearing 4T1 xenografts, and 70 kD FITC-Ficoll was injected into the tumors 1 h later. The mice were necropsied 1 h after Ficoll injection, and the tumors were excised and frozen in OCT.
RESULTS: EIPA-sensitive 70 kD FITC-Ficoll uptake was observed in in situ homozygous 4T1 tumors of BALB/c mice, suggesting that AMPK activation or other signals are sufficient to trigger the formation of large fusions in vivo. [1]
Synonyms
N-Acetyl-5-methoxytryptamine, Melatonine
Chemical Properties
Molecular Weight
232.28
Formula
C13H16N2O2
Cas No.
73-31-4
Smiles
O=C(NCCC1=CNC=2C=CC(OC)=CC21)C
Relative Density.
1.175g/cm3
Storage & Solubility Information
Storage
Solubility Information
In Vivo Formulation
10% DMSO+90% Saline: 1.16 mg/mL (4.99 mM), Solution. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
References
- 1.Liu H, et al. The effect and mechanisms of melatonin on the proliferation and apoptosis of lung cancer cells. Bioengineered. 2022 Feb;13(2):3462-3469.
- 2.Estaras M, et al. Melatonin controls cell proliferation and modulates mitochondrial physiology in pancreatic stellate cells. J Physiol Biochem. 2023 Feb;79(1):235-249.
- 3.Sugden D. Psychopharmacological effects of melatonin in mouse and rat. J Pharmacol Exp Ther. 1983 Dec;227(3):587-91.