NAD
Price range: $56.00 through $196.00
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Questions
NAD Common Questions
What’s pIC50?
pIC50 is the negative log of the IC50 value when converted to molar.That is, pIC50=-log(IC50).
What’s ED50?
Product Introduction
Bioactivity
Description
NAD+ (β-Nicotinamide Adenine Dinucleotide) is a coenzyme composed of ribosylnicotinamide 5′-diphosphate coupled to adenosine 5′-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH).
In vitro
METHODS: HEK293 cells were treated with FK866 (2 µM) and NAD+ (100 µM) for 48 h. Metabolic activity was determined by MTT Assay.
RESULTS: Addition of FK866 to the culture medium resulted in rapid depletion of intracellular NAD stores and inhibition of the metabolic activity of NADPH-dependent dehydrogenase. When supplemented with additional NAD+, the metabolic activity of the cells returned to control levels. [1]
METHODS: Isolated microvessels from rat retina were treated with NAD+ (0-1000 nM) for 0-24 h. Cell death was detected using trypan blue dye.
RESULTS: Exposure to NAD+ increased microvascular cell death in a dose-dependent manner, with the half-maximum effective concentration of NAD+ being approximately 2 nM. assessment of the time course of NAD+-induced vascular toxicity showed that cell death was detected after 16 h of NAD+ exposure. [2]
In vitro
METHODS: To study the effects on ischemia/reperfusion (I/R) injury, NAD+ (5-20 mg/kg) was injected intravenously into Wistar rats with myocardial ischemia/reperfusion.
RESULTS: Injections of 10-20 mg/kg NAD+ dose-dependently reduced I/R-induced myocardial infarction, with a dose of 20 mg/kg NAD+ reducing infarction by approximately 85%. Injection of NAD+ significantly reduced I/R-induced apoptotic cardiac injury. [3]
Synonyms
β-Nicotinamide Adenine Dinucleotide, β-NAD, β-DPN, DPN, Cozymase
Chemical Properties
Molecular Weight
663.43
Formula
C21H27N7O14P2
Cas No.
53-84-9
Smiles
O.NC(=O)c1ccc[n+](c1)[C@@H]1O[C@H](COP([O-])(=O)OP(O)(=O)OC[C@H]2O[C@H]([C@H](O)[C@@H]2O)n2cnc3c(N)ncnc23)[C@@H](O)[C@H]1O
Relative Density.
Ca. 1.6 g/cm3. Temperature:20 °C.
Storage & Solubility Information
Storage
Solubility Information
Solution Preparation Table
DMSO
References
- 1.Kulikova V, et al. Degradation of Extracellular NAD+ Intermediates in Cultures of Human HEK293 Cells. Metabolites. 2019 Nov 29;9(12):293.
- 2.Liao SD, et al. NAD+-induced vasotoxicity in the pericyte-containing microvasculature of the rat retina: effect of diabetes. Invest Ophthalmol Vis Sci. 2006 Nov;47(11):5032-8.
- 3.Zhang Y, et al. Exogenous NAD(+) administration significantly protects against myocardial ischemia/reperfusion injury in rat model. Am J Transl Res. 2016 Aug 15;8(8):3342-50.